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aquilos2 dual beam fib system  (Thermo Fisher)


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    Thermo Fisher aquilos2 dual beam fib system
    Aquilos2 Dual Beam Fib System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1953 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aquilos2 dual beam fib system/product/Thermo Fisher
    Average 96 stars, based on 1953 article reviews
    aquilos2 dual beam fib system - by Bioz Stars, 2026-03
    96/100 stars

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    Image Search Results


    Glutamate reprogram keloid fibroblasts transcripts. (A) Volcano plot show DEGs in HKF cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .

    Journal: Frontiers in Molecular Biosciences

    Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts

    doi: 10.3389/fmolb.2025.1720876

    Figure Lengend Snippet: Glutamate reprogram keloid fibroblasts transcripts. (A) Volcano plot show DEGs in HKF cells upon 100 μM glutamate stimulate for 24 h. (B,C) GO enrichment of DEGs (B) with bubble size representing the number of genes in each term and color indicating the adjusted q value, and KEGG enrichment of DEGs showing the top enriched signaling pathways related to cytokine and immune responses (C) . (D) Barplot of GSEA enrichment of DEGs, resented as normalized enrichment scores (NES); bars to the right indicate pathways positively enriched in GLU compared with PBS. (E,F) GSEA enrichment of cytokine-cytokine receptor interaction (E) and TGF beta signaling pathway (F) .

    Article Snippet: The HKF (Human keloid fibroblasts) cell line obtained from American Type Culture Collection (CRL-1762, Manassas, VA, United States), and were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Carlsbad, CA, United States) containing 10% fetal bovine serum (FBS; Life Technologies) at 37 °C in a 5% humidified CO 2 incubator.

    Techniques: Protein-Protein interactions

    Glutamate promotes inflammatory responses in keloid fibroblasts. (A–C) Volcano plot show DEGs that associated to cytokine activity pathway (A) , extracellular space pathway (B) , and inflammatory response pathway (C) in HKF cells upon 100 μM glutamate stimulate for 24 h. (D–F) Volcano plot and heatmap show DEGs that associated to TNF signaling pathway (D) , IL-17 signaling pathway (E) , and calcium signaling pathway (F) in HKF cells upon 100 μM glutamate stimulate for 24 h. (G) FPKM of inflammatory genes (CXCL8, CXCL3, CXCL2, IL11, IL6) of RNA-seq in keloid and normal tissues in HKF cells upon 100 μM glutamate stimulate for 24 h. (H) RT-PCR of inflammatory genes in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h. (I) ELISA of IL-6 andIL-11 in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts

    doi: 10.3389/fmolb.2025.1720876

    Figure Lengend Snippet: Glutamate promotes inflammatory responses in keloid fibroblasts. (A–C) Volcano plot show DEGs that associated to cytokine activity pathway (A) , extracellular space pathway (B) , and inflammatory response pathway (C) in HKF cells upon 100 μM glutamate stimulate for 24 h. (D–F) Volcano plot and heatmap show DEGs that associated to TNF signaling pathway (D) , IL-17 signaling pathway (E) , and calcium signaling pathway (F) in HKF cells upon 100 μM glutamate stimulate for 24 h. (G) FPKM of inflammatory genes (CXCL8, CXCL3, CXCL2, IL11, IL6) of RNA-seq in keloid and normal tissues in HKF cells upon 100 μM glutamate stimulate for 24 h. (H) RT-PCR of inflammatory genes in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h. (I) ELISA of IL-6 andIL-11 in HKF cells upon 10 μM or 100 μM glutamate stimulate for 24 h p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: The HKF (Human keloid fibroblasts) cell line obtained from American Type Culture Collection (CRL-1762, Manassas, VA, United States), and were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Carlsbad, CA, United States) containing 10% fetal bovine serum (FBS; Life Technologies) at 37 °C in a 5% humidified CO 2 incubator.

    Techniques: Activity Assay, RNA Sequencing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay